Candida albicans is the leading cause of fungal infections; yet, complex genetic interaction analysis remains cumbersome in this diploid pathogen. Here, we developed a CRISPR–Cas9-based ‘gene drive array’ platform to facilitate efficient genetic analysis in C. albicans. In our system, a modified DNA donor molecule acts as a selfish genetic element, replaces the targeted site and propagates to replace additional wild-type loci. Using mating-competent C. albicans haploids, each carrying a different gene drive disabling a gene of interest, we are able to create diploid strains that are homozygous double-deletion mutants. We generate double-gene deletion libraries to demonstrate this technology, targeting antifungal efflux and biofilm adhesion factors. We screen these libraries to identify virulence regulators and determine how genetic networks shift under diverse conditions. This platform transforms our ability to perform genetic interaction analysis in C. albicans and is readily extended to other fungal pathogens.
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We thank G. Fink, J. Berman, M. Hickman, V. Vyas and A. Baryshnikova for helpful discussions. We also thank V. Vyas, J. Köhler and L. Cowen for strains. This work was supported by the Paul G. Allen Frontiers Group, a Banting postdoctoral fellowship from the Canadian Institutes of Health Research, National Cancer Institute grantno. 5T32CA009216-34, US National Institutes of Health National Human Genome Research Institute grant no. RM1 HG008525 and the Wyss Institute for Biologically Inspired Engineering.
Electronic supplementary material
Supplementary Figures 1–4, Supplementary Figure legends, Supplementary Table legends and Supplementary Notes.
Gene drive construct variants. Related to Fig. 2. This table summarizes the different gene drive construct variants used as part of the optimization of the C. albicans gene drive system.Gene drive construct variants. Related to Fig. 2. This table summarizes the different gene drive construct variants used as part of the optimization of the C. albicans gene drive system.
C. albicans efflux and adhesin genes targeted for deletion, and library matrix summary. Related to Fig. 3. This table summarizes the different C. albicans adhesin and efflux genes targeted for deletion, and lists each single- and double-gene deletion strains generated as part of this study.C. albicans efflux and adhesin genes targeted for deletion, and library matrix summary. Related to Fig. 3. This table summarizes the different C. albicans adhesin and efflux genes targeted for deletion, and lists each single- and double-gene deletion strains generated as part of this study.
Whole-genome sequencing summary of gene drive deletion strains. Related to Figs. 2 and 3. This table summarizes the results of whole-genome sequencing, and lists each gene found to be deleted in different strain backgrounds, as well as sequence coverage information.
Genetic interaction scores and significant genetic interactions for double-gene deletion libraries. Related to Figs. 2–4. This table lists genetic interactions scores (calculated using a multiplicative model) and significant positive and negative genetic interactions for both C. albicans double-gene deletion libraries (efflux and adhesin mutants).
Summary of antifungal perturbations for drug efflux pump deletion screening. Related to Fig. 4. This table lists all perturbation conditions used for screening the C. albicans efflux pump library, including the concentration of drug tested in the screen.
Table of gene drive construct variants and optimization.
About this article
Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in S. cerevisiae
Fungal Biology and Biotechnology (2019)
Design, execution, and analysis of CRISPR–Cas9-based deletions and genetic interaction networks in the fungal pathogen Candida albicans
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