The major difference between viruses and plasmids is the mechanism of transferring their genomic information between host cells. Here, we describe the archaeal plasmid pR1SE from an Antarctic species of haloarchaea that transfers via a mechanism similar to a virus. pR1SE encodes proteins that are found in regularly shaped membrane vesicles, and the vesicles enclose the plasmid DNA. The released vesicles are capable of infecting a plasmid-free strain, which then gains the ability to produce plasmid-containing vesicles. pR1SE can integrate and replicate as part of the host genome, resolve out with fragments of host DNA incorporated or portions of the plasmid left behind, form vesicles and transfer to new hosts. The pR1SE mechanism of transfer of DNA could represent the predecessor of a strategy used by viruses to pass on their genomic DNA and fulfil roles in gene exchange, supporting a strong evolutionary connection between plasmids and viruses.
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This work was supported by the Australian Research Council (DP150100244) and the Australian Antarctic Science program (project 4031). S.E. was supported by the EMBO Long-Term Fellowship ALTF 188–2014, which is co-funded by the European Commission (EMBOCOFUND2012, GA-2012-600394) and supported by Marie Curie Actions. Mass spectrometry results were obtained at the Bioanalytical Mass Spectrometry Facility (BMSF) and electron microscopy at the Electron Microscope Unit, both within the Analytical Centre of the University of New South Wales. Subsidized access to the BMSF is acknowledged. The authors thank the PRIDE team and ProteomeXchange for efficiently processing and hosting the mass spectrometry data. The authors thank A. Hancock for providing the image of Rauer 1 Lake, the Landsat Image Mosaic of Antarctica (LIMA) project for making satellite images available, S. Payne and A. Hancock for collecting Antarctic water samples, M. Allen for providing uncharacterized strains of Hrr. lacusprofundi, R. Kuchel for assistance with electron microscopy, D. Baker and I. Anishchanka for attempting structural predictions and T. Williams for comments about the manuscript.
Electronic supplementary material
Supplementary Notes, Supplementary References, Supplementary Figures and Supplementary Tables.
pR1SEDL18 contig with annotation
All proteins detected in vesicle and membrane preparations.
All proteins detected in PVs purified by CsCl gradient centrifugation.
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Nature Microbiology (2017)