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PROTEIN PATTERNING

Moving hydrogels to the fourth dimension

Materials that permit spatiotemporal control of biomolecule presentation have long been a challenge in the field. A method has now been developed to reversibly pattern cell-laden hydrogels with site-specifically immobilized proteins using sortase-mediated transpeptidation without compromising bioactivity.

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Fig. 1: Photopatterning and photorelease of sortase-tag enhanced protein ligation (STEPL)-modified proteins in hydrogels.

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Correspondence to Jason A. Burdick.

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Galarraga, J.H., Burdick, J.A. Moving hydrogels to the fourth dimension. Nat. Mater. 18, 914–915 (2019). https://doi.org/10.1038/s41563-019-0458-5

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