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Non-specific interactions govern cytosolic diffusion of nanosized objects in mammalian cells

Nature Materialsvolume 17pages740746 (2018) | Download Citation

Abstract

The diffusivity of macromolecules in the cytoplasm of eukaryotic cells varies over orders of magnitude and dictates the kinetics of cellular processes. However, a general description that associates the Brownian or anomalous nature of intracellular diffusion to the architectural and biochemical properties of the cytoplasm has not been achieved. Here we measure the mobility of individual fluorescent nanoparticles in living mammalian cells to obtain a comprehensive analysis of cytoplasmic diffusion. We identify a correlation between tracer size, its biochemical nature and its mobility. Inert particles with size equal or below 50 nm behave as Brownian particles diffusing in a medium of low viscosity with negligible effects of molecular crowding. Increasing the strength of non-specific interactions of the nanoparticles within the cytoplasm gradually reduces their mobility and leads to subdiffusive behaviour. These experimental observations and the transition from Brownian to subdiffusive motion can be captured in a minimal phenomenological model.

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Change history

  • 19 September 2018

    In the version of this Article originally published, Supplementary Videos 3–5 were incorrectly labelled; 3 should have been 5, 4 should have been 3 and 5 should have been 4. This has now been corrected.

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Acknowledgements

We acknowledge M. Coppey-Moisan, B. Goud and S. Letard and P. Dubreuil for supplying various cells (see text). E.B. acknowledges the Ecole Doctorale Frontières du Vivant (FdV)—Programme Bettencourt for financial support. M.D. and M.C. acknowledge financial support from the French National Research Agency (ANR) Paris-Science-Lettres Program (ANR-10-IDEX-0001-02 PSL), Labex CelTisPhyBio (ANR-10-LBX-0038), the Human Frontier Science Program (grant no. RGP0005/2007) and the France-BioImaging infrastructure supported by ANR Grant ANR-10-INSB-04 (Investments for the Future). This project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement no. 686841 (MAGNEURON).

Author information

Author notes

    • Fred Etoc

    Present address: Center for Studies in Physics and Biology, The Rockefeller University, New York, NY, USA

    • Chiara Vicario

    Present address: Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain

  1. These authors contributed equally: Elie Balloul, Chiara Vicario.

Affiliations

  1. Laboratoire Physico-Chimie, Institut Curie, CNRS UMR168, PSL Research University, Université Pierre et Marie Curie-Paris, Paris, France

    • Fred Etoc
    • , Elie Balloul
    • , Chiara Vicario
    • , Davide Normanno
    • , Maxime Dahan
    •  & Mathieu Coppey
  2. Centre de Recherche en Cancérologie de Marseille, CNRS UMR7258, Inserm U1068, Aix-Marseille Université UM105, Institut Paoli-Calmettes, Marseilles, France

    • Davide Normanno
  3. Division of Biophysics, Department of Biology, Osnabrück University, Osnabrück, Germany

    • Domenik Liße
    •  & Jacob Piehler
  4. Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel

    • Assa Sittner

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Contributions

F.E., M.D. and M.C. conceived the study. F.E. performed experiments and numerical simulations. F.E. and D.N. did the ferritin NP experiments in HeLa cells. A.S. executed the early experiments. C.V. conducted the NP-release validation experiments. E.B. performed the RPE-1 and hMSC experiments. D.N. collected HDFa data. D.L. and J.P. provided reagents. F.E., E.B. and M.C. analysed the data. All the authors discussed the results and commented on the manuscript. F.E., D.N., M.D. and M.C. wrote the manuscript.

Competing interests

The authors declare no competing interests.

Corresponding authors

Correspondence to Davide Normanno or Maxime Dahan or Mathieu Coppey.

Supplementary information

  1. Supplementary Information

    Supplementary Video Legends 1–5, Supplementary Figures 1–17, Supplementary Tables 1–3

  2. Reporting Summary

    Life Sciences Reporting Summary

  3. Supplementary Video 1

    Representative video of 25 nm Rho-NPs diffusing in a HeLa cell after pinocytic loading. Three unburst vesicles can be seen at the top of the images: two very, close together, on the left and one, very bright, in the middle. These unburst vesicles are much larger than individual NPs and are basically immobile. Images were acquired every 10 ms, the video is encoded at 30 frames per second (fps). Scale bar represents 10 μm

  4. Supplementary Video 2

    Representative videos of 25 nm Rho-NPs diffusing in HeLa cells after pinocytic loading. Top row: crops of the field of view showing unburst vesicles. NPs encapsulated within these intact vesicles move very fast and cannot be tracked individually. Bottom row: examples of typical regions of interest chosen for NPs single-particle tracking analysis and selected in a way such that unburst vesicles were systematically excluded. The marked difference in the behaviour of free versus encapsulated NPs ensures a reliable discrimination between the two cases. Images were acquired every 10 ms, the video is encoded at 30 fps. Scale bars represent 1 μm

  5. Supplementary Video 3

    Representative video of QDs (QDs-PEG-NH2) diffusing in a HeLa cell after pinocytic loading. QDs clearly show on-off fluctuations (blinking) of the fluorescence signal (use Supplementary Fig. 4 as reference to more easily spot blinking particles). The blinking behaviour is a typical signature of individual emitters and indicates that QDs are mono-dispersed, confirming their escape from pinocytic vesicles and dismissing potential aggregations. Images were acquired every 30 ms, the video is encoded at 15 fps. The counter indicates the frame number. Scale bar represents 2 μm

  6. Supplementary Video 4

    Representative video of the simultaneous diffusion of two different types of QDs, QDs-605 emitting at 605 nm (green) and QDs-PEG-NH2 emitting at 655 nm (red), after concomitant pinocytic internalization. The images clearly illustrate that in the two HeLa cells visible in the video there is no co-localization of the two probes, on the contrary of what expect if NPs would have been still encapsulated inside unburst vesicles. Images were acquired every 30 ms, the video is encoded at 15 fps. Scale bar represents 5 μm

  7. Supplementary Video 5

    The video shows the concurrent detection of the two types of QDs displayed in Supplementary Video 4: QDs-605 (red circles) and QDs-PEG-NH2 (yellow circles). The images put well in evidence the absence of correlated dynamics in the mobility of the two probes, on the contrary of what expected if NPs would have been still encapsulated inside unburst vesicles. Images were acquired every 30 ms, the video is encoded at 10 fps. Scale bar represents 5 μm

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DOI

https://doi.org/10.1038/s41563-018-0120-7

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