Females that are highly selective when choosing a mate run the risk of remaining unmated or delaying commencing reproduction. Therefore, low female choosiness would be beneficial when males are rare but it would be maladaptive if males become more frequent. How can females resolve this issue? Polyandry would allow mating-status-dependent choosiness, with virgin females selecting their first mate with little selectivity and becoming choosier thereafter. This plasticity in choosiness would ensure timely acquisition of sperm and enable females to increase offspring quality during later mating. Here, we show that Drosophila melanogaster females display such mating-status-dependent choosiness by becoming more selective once mated and identify the underlying neurohormonal mechanism. Mating releases juvenile hormone, which desensitizes Or47b olfactory neurons to a pheromone produced by males, resulting in increased preference for pheromone-rich males. Besides providing a mechanism to a long-standing evolutionary prediction, these data suggest that intersexual selection in D. melanogaster, and possibly in all polyandrous, sperm-storing species, is mainly the domain of mated females since virgin females are less selective. Juvenile hormone influences behaviour by changing cue responsiveness across insects; the neurohormonal modulation of olfactory neurons uncovered in D. melanogaster provides an explicit mechanism for how this hormone modulates behavioural plasticity.
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We thank J. Wang for critical advice on the experiments and manuscript and M. Maan, T. Verschut and M. Laturney for feedback on the manuscript. This work was supported in part by a grant from the Dutch organization for scientific research (no. ALWOP.611) to J.-C.B., a fellowship by the German Academic Exchange Service (no. 57386478) and a fellowship of the German National Academy of Sciences Leopoldina (no. LPDS 2018-11) to P.K. and grants from the National Institutes of Health to C.-Y.S. (nos. R01DC016466, R01DC015519 and R21DC108912).
The authors declare no competing interests.
Peer review information Nature Ecology & Evolution thanks Mariana Wolfner, Jen Perry and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
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Extended Data Fig. 1 Mating latency is influenced by an interaction between female mating status and male genotype.
Canton-S virgin females were offered a single male (Nl or Tai) for mating. After mating, females were isolated for 24h and offered a new single male (Nl or Tai) for remating. Virgin and mated female mating latency was recorded. PLots display (Mean ± s.e.m). A generalized linear mixed model including mating latency as a response variable, male genotype (Nl vs. Tai), female mating status (virgin, mated) and their interaction as explanatory factors and female ID as a random factor revealed that mating latency is influenced by an interaction between male genotype and mating status (GLMM: p = 0.038). The interaction was then explored using posthoc tests.
Normalized and log transformed read counts of antennal transcriptomes separated by mating status. Isoforms marked with (*) were differentially expressed between virgin and mated females (Mean ± s.e.m). *: p<0.05; **:p<0.01; ***:p<0.001.
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Kohlmeier, P., Zhang, Y., Gorter, J.A. et al. Mating increases Drosophila melanogaster females’ choosiness by reducing olfactory sensitivity to a male pheromone. Nat Ecol Evol 5, 1165–1173 (2021). https://doi.org/10.1038/s41559-021-01482-4
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