To function properly, mitochondria utilize products of 37 mitochondrial and >1,000 nuclear genes, which should be compatible with each other. Discordance between mitochondrial and nuclear genetic ancestry could contribute to phenotypic variation in admixed populations. Here, we explored potential mitonuclear incompatibility in six admixed human populations from the Americas: African Americans, African Caribbeans, Colombians, Mexicans, Peruvians and Puerto Ricans. By comparing nuclear versus mitochondrial ancestry in these populations, we first show that mitochondrial DNA (mtDNA) copy number decreases with increasing discordance between nuclear and mtDNA ancestry. The direction of this effect is consistent across mtDNA haplogroups of different geographic origins. This observation indicates suboptimal regulation of mtDNA replication when its components are encoded by nuclear and mtDNA genes with different ancestry. Second, while most populations analysed exhibit no such trend, in African Americans and Puerto Ricans, we find a significant enrichment of ancestry at nuclear-encoded mitochondrial genes towards the source populations contributing the most prevalent mtDNA haplogroups (African and Native American, respectively). This possibly reflects compensatory effects of selection in recovering mitonuclear interactions optimized in the source populations. Our results provide evidence of mitonuclear interactions in human admixed populations and we discuss their implications for human health and disease.
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Frontiers in Zoology Open Access 06 September 2021
Disrupted architecture and fast evolution of the mitochondrial genome of Argeia pugettensis (Isopoda): implications for speciation and fitness
BMC Genomics Open Access 03 September 2020
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All analyses were conducted using publicly available data. The 1000 Genomes Project data are available on its FTP site. Intermediate files and code have been made publicly available on github: (https://github.com/makovalab-psu/Mito_nuclear_incompatibility).
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We thank R. Nielsen, M. Shriver, W. Chase and S. Craig for their comments on the manuscript. We would also like to thank E. Torres Gonzalez for reviewing the code. This project was supported by a seed grant awarded to A.A.Z. and K.D.M. from the Center of Human Evolution and Diversity (CHED) at The Pennsylvania State University and by a grant from NIH (R01GM116044). Additional funding was provided by the Eberly College of Sciences, The Huck Institute of Life Sciences and the Institute for CyberScience at Penn State, as well as, in part, under grants from the Pennsylvania Department of Health using Tobacco Settlement and CURE Funds. The department specifically disclaims any responsibility for any analyses, responsibility or conclusions.
The authors declare no competing interests.
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Supplementary Figures 1–11
Supplementary Table 1, mtDNA copy number data; Supplementary Table 2, Autosomal ancestry fractions for all samples used in the study; Supplementary Table 3, X-chromosomal ancestry fractions for all samples used in the study; Supplementary Table 4, mtDNA haplogroup information for each sample; Supplementary Table 5, List of genes from Mitocarta 2.0 with functional annotation
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Zaidi, A.A., Makova, K.D. Investigating mitonuclear interactions in human admixed populations. Nat Ecol Evol 3, 213–222 (2019). https://doi.org/10.1038/s41559-018-0766-1
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