Recent genomic analyses show that the earliest peoples reaching Remote Oceania—associated with Austronesian-speaking Lapita culture—were almost completely East Asian, without detectable Papuan ancestry. However, Papuan-related genetic ancestry is found across present-day Pacific populations, indicating that peoples from Near Oceania have played a significant, but largely unknown, ancestral role. Here, new genome-wide data from 19 ancient South Pacific individuals provide direct evidence of a so-far undescribed Papuan expansion into Remote Oceania starting ~2,500 yr bp, far earlier than previously estimated and supporting a model from historical linguistics. New genome-wide data from 27 contemporary ni-Vanuatu demonstrate a subsequent and almost complete replacement of Lapita-Austronesian by Near Oceanian ancestry. Despite this massive demographic change, incoming Papuan languages did not replace Austronesian languages. Population replacement with language continuity is extremely rare—if not unprecedented—in human history. Our analyses show that rather than one large-scale event, the process was incremental and complex, with repeated migrations and sex-biased admixture with peoples from the Bismarck Archipelago.
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Clarkson, C. et al. Human occupation of northern Australia by 65,000 years ago. Nature 547, 306–310 (2017).
Pawley, A. & Green, R. Dating the dispersal of the Oceanic languages. Ocean. Linguist. 12, 1–67 (1973).
Kirch, P. V. On the Road of the Winds: An Archaeological History of the Pacific Islands Before European Contact (Univ. California Press, Berkeley, 2017).
Blust, R. A. The prehistory of the Austronesian-speaking peoples: a view from language. J. World Prehist. 9, 453–510 (1995).
Gray, R. D., Drummond, A. J. & Greenhill, S. J. Language phylogenies reveal expansion pulses and pauses in Pacific settlement. Science 323, 479–483 (2009).
Blust, R. A. The Austronesian Languages (Australian National Univ., Canberra, 2009).
Summerhayes, G. R. et al. Tamuarawai (EQS): an early Lapita site on Emirau, New Ireland, PNG. J. Pac. Archaeol. 1, 62–75 (2010).
Sheppard, P. J. Lapita colonization across the Near/Remote Oceania boundary. Curr. Anthropol. 52, 799–840 (2011).
Pugach, I. et al. The gateway from Near into Remote Oceania: new insights from genome-wide data. Mol. Biol. Evol. https://doi.org/10.1093/molbev/msx333 (2018).
Petchey, F. J., Spriggs, M., Bedford, S., Valentin, F. & Buckley, H. Radiocarbon dating of burials from the Teouma Lapita cemetery, Efate, Vanuatu. J. Archaeol. Sci. 50, 227–242 (2014).
Sand, C. Lapita Calédonien. Archéologie d’un Premier Peuplement Insulaire Océanien (Société des Océanistes, Paris, 2010).
Burley, D., Weisler, M. I. & Zhao, J.-X. High precision U/Th dating of first Polynesian settlement. PLoS ONE 7, e48769 (2012).
Wollstein, A. et al. Demographic history of Oceania inferred from genome-wide data. Curr. Biol. 20, 1983–1992 (2010).
Pawley, A., Attenborough, R., Golson, J. & Hide, R. Papuan Pasts: Cultural, Linguistic and Biological Histories of Papuan-Speaking Peoples (Australian National Univ., Canberra, 2005).
Lynch, J., Ross, M. & Crowley, T. The Oceanic Languages (Curzon, Richmond, 2002).
Kayser, M. et al. Genome-wide analysis indicates more Asian than Melanesian ancestry of Polynesians. Am. J. Hum. Genet. 82, 194–198 (2008).
Melton, T. et al. Polynesian genetic affinities with Southeast Asian populations as identified by mtDNA analysis. Am. J. Hum. Genet. 57, 403–414 (1995).
Kayser, M. et al. Melanesian origin of Polynesian Y chromosomes. Curr. Biol. 10, 1237–1246 (2000).
Hurles, M. E. et al. Y-chromosomal evidence for the origins of Oceanic-speaking peoples. Genetics 160, 289–303 (2002).
Kayser, M. et al. Melanesian and Asian origins of Polynesians: mtDNA and Y chromosome gradients across the Pacific. Mol. Biol. Evol. 23, 2234–2244 (2006).
Skoglund, P. et al. Origins and genetic legacy of the first people in Remote Oceania. Nature 538, 510–513 (2016).
Valentin, F., Détroit, F., Spriggs, M. & Bedford, S. Early Lapita skeletons from Vanuatu show Polynesian craniofacial shape: implications for Remote Oceanic settlement and Lapita origins. Proc. Natl Acad. Sci. USA 113, 292–297 (2015).
Bedford, S. & Spriggs, M. in The Oxford Handbook of Prehistoric Oceania (eds Cochrane, E. & Hunt, T.) Ch. 8 (Oxford Univ. Press, Oxford, 2014).
Dabney, J. et al. Complete mitochondrial genome sequence of a Middle Pleistocene cave bear reconstructed from ultrashort DNA fragments. Proc. Natl Acad. Sci. USA 110, 15758–15763 (2013).
Meyer, M. & Kircher, M. Illumina sequencing library preparation for highly multiplexed target capture and sequencing. Cold Spring Harb. Protoc. 2010, pdb.prot5448 (2010).
Rohland, N., Harney, E., Mallick, S., Nordenfelt, S. & Reich, D. Partial uracil–DNA–glycosylase treatment for screening of ancient DNA. Phil. Trans. R. Soc. B 370, 20130624 (2015).
Fu, Q. et al. A revised timescale for human evolution based on ancient mitochondrial genomes. Curr. Biol. 23, 553–559 (2013).
Fu, Q. et al. An early modern human from Romania with a recent Neanderthal ancestor. Nature 524, 216–219 (2015).
Key, F. M., Posth, C., Krause, J., Herbig, A. & Bos, K. I. Mining metagenomic data sets for ancient DNA: recommended protocols for authentication. Trends Genet. 33, 508–520 (2017).
Patterson, N. et al. Ancient admixture in human history. Genetics 192, 1065–1093 (2012).
Mallick, S. et al. The Simons Genome Diversity Project: 300 genomes from 142 diverse populations. Nature 538, 201–206 (2016).
Parks, T. et al. Association between a common immunoglobulin heavy chain allele and rheumatic heart disease risk in Oceania. Nat. Commun. 8, 14946 (2017).
Loh, P. R. et al. Inferring admixture histories of human populations using linkage disequilibrium. Genetics 193, 1233–1254 (2013).
Alexander, D. H., Novembre, J. & Lange, K. Fast model-based estimation of ancestry in unrelated individuals. Genome Res. 19, 1655–1664 (2009).
Reich, D. et al. Reconstructing Native American population history. Nature 488, 370–374 (2012).
Haak, W. et al. Massive migration from the steppe was a source for Indo-European languages in Europe. Nature 522, 207–211 (2015).
Reich, D. et al. Denisova admixture and the first modern human dispersals into Southeast Asia and Oceania. Am. J. Hum. Genet. 89, 516–528 (2011).
Mirabal, S. et al. Increased Y-chromosome resolution of haplogroup O suggests genetic ties between the Ami aborigines of Taiwan and the Polynesian Islands of Samoa and Tonga. Gene 492, 339–348 (2012).
Oliver, D. L. Ancient Tahitian Society: Ethnography (Univ. Press of Hawaii, Honolulu, 1974).
Wilmshurst, J. M., Hunt, T. L., Lipo, C. P. & Anderson, A. J. High-precision radiocarbon dating shows recent and rapid initial human colonization of East Polynesia. Proc. Natl Acad. Sci. USA 108, 1815–1820 (2011).
Blust, R. Review of Lynch, Ross, and Crowley, “The Oceanic Languages”. Ocean. Linguist. 44, 544–548 (2005).
Blust, R. Remote Melanesia: one history or two? An addendum to Donohue and Denham. Ocean. Linguist. 47, 445–459 (2008).
Donohue, M. & Denham, T. The language of Lapita: Vanuatu and an early Papuan presence in the Pacific. Ocean. Linguist. 47, 365–376 (2008).
Lynch, J. Melanesian diversity and Polynesian homogeneity: the other side of the coin. Ocean. Linguist. 20, 95–129 (1981).
Tryon, D. T. in Melanesia: Beyond Diversity (eds May, R. J. & Nelson, H.) 241–248 (Australian National Univ., Canberra, 1982).
Speiser, F. Ethnology of Vanuatu. An Early Twentieth Century Study (Crawford House Press, Bathurst, 1996).
Pawley, A. Explaining the aberrant Austronesian languages of Southeast Melanesia: 150 years of debate. J. Polyn. Soc. 115, 215–258 (2006).
Clark, R. Leo Tuai: a Comparative Lexical Study of North and Central Vanuatu Languages (Australian National Univ., Canberra, 2009).
Lynch, J. The Linguistic History of Southern Vanuatu (Australian National Univ., Canberra, 2001).
Bedford, S. Pieces of the Vanuatu Puzzle: Archaeology of the North, South and Centre Vol. 23 157–192 (Australian National Univ., Canberra, 2006).
Rohland, N. & Hofreiter, M. Ancient DNA extraction from bones and teeth. Nat. Protoc. 2, 1756–1762 (2007).
Kircher, M., Sawyer, S. & Meyer, M. Double indexing overcomes inaccuracies in multiplex sequencing on the Illumina platform. Nucleic Acids Res. 40, e3 (2012).
Fu, Q. et al. DNA analysis of an early modern human from Tianyuan Cave, China. Proc. Natl Acad. Sci. USA 110, 2223–2227 (2013).
Peltzer, A. et al. EAGER: efficient ancient genome reconstruction. Genome Biol. 17, 60 (2016).
Schubert, M., Lindgreen, S. & Orlando, L. AdapterRemoval v2: rapid adapter trimming, identification, and read merging. BMC Res. Notes 9, 88 (2016).
Li, H. & Durbin, R. Fast and accurate short read alignment with Burrows–Wheeler transform. Bioinformatics 25, 1754–1760 (2009).
Li, H. et al. The Sequence Alignment/Map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).
Skoglund, P. et al. Separating endogenous ancient DNA from modern day contamination in a Siberian Neandertal. Proc. Natl Acad. Sci. USA 111, 2229–2234 (2014).
Fu, Q. et al. The genetic history of Ice Age Europe. Nature 534, 200–205 (2016).
Korneliussen, T. S., Albrechtsen, A. & Nielsen, R. ANGSD: analysis of next generation sequencing data. BMC Bioinformatics 15, 356 (2014).
Meyer, M. et al. A mitochondrial genome sequence of a hominin from Sima de los Huesos. Nature 505, 403–406 (2014).
Renaud, G., Slon, V., Duggan, A. T. & Kelso, J. schmutzi: estimation of contamination and endogenous mitochondrial consensus calling for ancient DNA. Genome Biol. 16, 224 (2015).
Price, A. L. et al. Principal components analysis corrects for stratification in genome-wide association studies. Nat. Genet. 38, 904–909 (2006).
Moorjani, P. et al. A genetic method for dating ancient genomes provides a direct estimate of human generation interval in the last 45,000 years. Proc. Natl Acad. Sci. USA 113, 5652–5657 (2016).
Reich, D., Thangaraj, K., Patterson, N., Price, A. L. & Singh, L. Reconstructing Indian population history. Nature 461, 489–494 (2009).
Reich, D. et al. Genetic history of an archaic hominin group from Denisova Cave in Siberia. Nature 468, 1053–1060 (2010).
Weissensteiner, H. et al. HaploGrep 2: mitochondrial haplogroup classification in the era of high-throughput sequencing. Nucleic Acids Res. 44, W58–W63 (2016).
We thank the communities in Malakula and Efate in Vanuatu who participated in this study, and particularly all sample donors. We are grateful to M. Stoneking, I. Pugach and C.-C. Wang for comments, and to G. Brandt, R. Bianco and technicians at the Max Planck Institute for the Science of Human History for laboratory support. This research was supported by the Max Planck Society. Archaeological investigations on Malakula, Vanuatu were funded by the Sasakawa Pacific Island Nations Fund, the Marsden Fund of the Royal Society of New Zealand (Fast-Start 9011/3602128; 04-U00–007), a National Geographic Scientific Research grant (7738–04) and an Australian Research Council Discovery Project grant (DP0880789). Investigations on Tanna, Vanuatu were supported by an Australian Research Council Discover Project grant (DP160103578). F.V. is funded by CNRS-UMR 7041, H.B. is funded by the Marsden Fund of the Royal Society of New Zealand (Standard Grant UOO0917) and a University of Otago Research Grant, and A.P. is funded by European Research Council Starting Grant ‘Waves’ (ERC758967).
The authors declare no competing interests.
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Posth, C., Nägele, K., Colleran, H. et al. Language continuity despite population replacement in Remote Oceania. Nat Ecol Evol 2, 731–740 (2018). https://doi.org/10.1038/s41559-018-0498-2
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