The phenomenon of de novo gene birth from junk DNA is surprising, because random polypeptides are expected to be toxic. There are two conflicting views about how de novo gene birth is nevertheless possible: the continuum hypothesis invokes a gradual gene birth process, whereas the preadaptation hypothesis predicts that young genes will show extreme levels of gene-like traits. We show that intrinsic structural disorder conforms to the predictions of the preadaptation hypothesis and falsifies the continuum hypothesis, with all genes having higher levels than translated junk DNA, but young genes having the highest level of all. Results are robust to homology detection bias, to the non-independence of multiple members of the same gene family and to the false positive annotation of protein-coding genes.
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Work was supported by the John Templeton Foundation (39667), the National Institutes of Health (GM104040) and ERC grant NewGenes (322564). We thank D. Tautz and M. Cordes for discussions, R. Bakaric for assistance with phylostratigraphy and A.-R. Carvunis for comments on a draft of the manuscript and for sharing data.
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Supplementary Figure 1 and Supplementary Table 1 (PDF 376 kb)
M. musculus proteins. (CSV 12497 kb)
Nucleotide sequences from intergenic regions of M. musculus genome (CSV 70010 kb)
Nucleotide sequences from intergenic regions of the masked M. musculus genome (CSV 70154 kb)
Randomly generated nucleotide sequences (CSV 35221 kb)
Scrambled amino acid sequences (CSV 12119 kb)
S. cerevisiae proteins from Table 1 (CSV 3330 kb)
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Wilson, B., Foy, S., Neme, R. et al. Young genes are highly disordered as predicted by the preadaptation hypothesis of de novo gene birth. Nat Ecol Evol 1, 0146 (2017). https://doi.org/10.1038/s41559-017-0146
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