Urbanization erodes ectomycorrhizal fungal diversity and may cause microbial communities to converge

Abstract

Urbanization alters the physicochemical environment, introduces non-native species and causes ecosystem characteristics to converge. It has been speculated that these alterations contribute to loss of regional and global biodiversity, but so far most urban studies have assessed macro-organisms and reported mixed evidence for biodiversity loss. We studied five cities on three continents to assess the global convergence of urban soil microbial communities. We determined the extent to which communities of bacteria, archaea and fungi are geographically distributed, and to what extent urbanization acts as a filter on species diversity. We discovered that microbial communities in general converge, but the response differed among microbial domains; soil archaeal communities showed the strongest convergence, followed by fungi, while soil bacterial communities did not converge. Our data suggest that urban soil archaeal and bacterial communities are not vulnerable to biodiversity loss, whereas urbanization may be contributing to the global diversity loss of ectomycorrhizal fungi. Ectomycorrhizae decreased in both abundance and species richness under turf and ruderal land-uses. These data add to an emerging pattern of widespread suppression of ectomycorrhizal fungi by human land-uses that involve physical disruption of the soil, management of the plant community, or nutrient enrichment.

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Figure 1: Geographic distribution of cities sampled, edaphic co-factors and community composition.
Figure 2: Effect of land-use on beta diversity, abundance and alpha diversity of microbial domains.
Figure 3: Within-group multivariate dispersal by grouping variable in each domain.
Figure 4: Relationship bewtween land-use, and ECM diversity and abundance.

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Acknowledgements

This research was funded through NSF ACI 1244820; soil chemical analysis was funded through SZIE-ÁOTK: KK-UK-12007. We acknowledge G. Dlott for technical support in QIIME, and R. E. Draskovits, S. Molnar, E. Powell, M. Bernard, Z. Toth and S. Mishra assisted with field sampling.

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D.J.E.S. constructed the DNA and sequencing libraries, and conducted Q-PCR, bioinformatics processing and statistical analyses. K.S. is the PI of the grant, designed the study and selected the sites in Baltimore. R.V.P., H.S., D.J.K., E.H., S.C. and I.Y. designed the study, selected the sites and participated in soil sampling. M.D. participated in soil sampling and provided nutrient data on soils; S.A.Y. designed the study, and oversaw all of the lab work, bioinformatics and data analysis. All authors discussed results and commented on the manuscript.

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Correspondence to Stephanie A. Yarwood.

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The authors declare no competing financial interests.

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Epp Schmidt, D., Pouyat, R., Szlavecz, K. et al. Urbanization erodes ectomycorrhizal fungal diversity and may cause microbial communities to converge. Nat Ecol Evol 1, 0123 (2017). https://doi.org/10.1038/s41559-017-0123

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