(a) Genic regions are significantly more frequently methylated than intergenic regions (3.9% versus 3.0%; Fisher’s exact P < 10-300). (b) Methylation levels are bimodally distributed in exons, introns and intergenic regions. Exons have the highest methylation levels, followed by introns and intergenic regions. (c) Relative frequencies of methylated positions across a standardized gene model with flanking 4 kb regions indicate that methylated positions are more frequently found at both ends of the gene model. Solid lines depict transcriptional start site (left) and transcription termination site (right) while dotted lines delineate the borders of the indicated genomic feature. The widths of the features correspond to mean normalized lengths of the respective exons and introns in P. daedalea (exons, from left to right: 286 bp, 320 bp, 225 bp, 203 bp, 270 bp, 380 bp; introns, from left to right: 1,971 bp, 1,744 bp, 1,598 bp, 1,728 bp).