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BIOCONJUGATES

Decorating proteins with LACE

Labelling proteins at internal sites holds promise for generating novel protein conjugates in a programmable fashion. Now, a chemoenzymatic approach, dubbed LACE, enables the site-specific modification of recombinant proteins that contain a short genetically encoded tag.

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Fig. 1: Labelling proteins at internal sites via chemoenzymatic approaches.

References

  1. 1.

    Boutureira, O. & Bernardes, G. J. Chem. Rev. 115, 2174–2195 (2015).

    CAS  Article  Google Scholar 

  2. 2.

    Lang, K. & Chin, J. W. Chem. Rev. 114, 4764–4806 (2014).

    CAS  Article  Google Scholar 

  3. 3.

    Zhang, Y., Park, K. Y., Suazo, K. F. & Distefano, M. D. Chem. Soc. Rev. 47, 9106–9136 (2018).

    CAS  Article  Google Scholar 

  4. 4.

    Lotze, J., Reinhardt, U., Seitz, O. & Beck-Sickinger, A. G. Mol. Biosyst. 12, 1731–1745 (2016).

    CAS  Article  Google Scholar 

  5. 5.

    Hofmann, R., Akimoto, G., Wucherpfennig, T. G., Zeymer, C. & Bode, K. W. Nat. Chem. https://doi.org/10.1038/s41557-020-0528-y (2020).

  6. 6.

    Komander, D. & Rape, M. Annu. Rev. Biochem. 81, 203–229 (2012).

    CAS  Article  Google Scholar 

  7. 7.

    Bernier-Villamor, V., Sampson, D. A., Matunis, M. J. & Lima, C. D. Cell 108, 345–356 (2002).

    CAS  Article  Google Scholar 

  8. 8.

    Mali, S. M., Singh, S. K., Eid, E. & Brik, A. J. Am. Chem. Soc. 139, 4971–4986 (2017).

    CAS  Article  Google Scholar 

  9. 9.

    Fottner, M. et al. Nat. Chem. Biol. 15, 276–284 (2019).

    CAS  Article  Google Scholar 

Download references

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Correspondence to Kathrin Lang.

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Fottner, M., Lang, K. Decorating proteins with LACE. Nat. Chem. 12, 980–982 (2020). https://doi.org/10.1038/s41557-020-00566-1

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