Protein engineering through tandem transamidation

Abstract

Semisynthetic proteins engineered to contain non-coded elements such as post-translational modifications (PTMs) represent a powerful class of tools for interrogating biological processes. Here, we introduce a one-pot, chemoenzymatic method that allows broad access to chemically modified proteins. The approach involves a tandem transamidation reaction cascade that integrates intein-mediated protein splicing with enzyme-mediated peptide ligation. We show that this approach can be used to introduce PTMs and biochemical probes into a range of proteins including Cas9 nuclease and the transcriptional regulator MeCP2, which causes Rett syndrome when mutated. The versatility of the approach is further illustrated through the chemical tailoring of histone proteins within a native chromatin setting. We expect our approach will extend the scope of semisynthesis in protein engineering.

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Fig. 1: Protein semisynthesis by TAIL.
Fig. 2: Identification of mutant inteins for use in TAIL.
Fig. 3: C-terminal protein engineering using TAIL (C-TAIL).
Fig. 4: N-terminal protein engineering using TAIL (N-TAIL).
Fig. 5: Application of TAIL to protein modification in increasingly complex environments.

Data availability

All relevant data are included in the Supplementary Information and are available from the authors. Plasmids for the following vectors have been deposited, along with maps and sequences, in Addgene: pET30-His6-Sumo-IntNtr-eSrt2A, Addgene plasmid #126015; pET30-His6-Sumo-MBP-CfaN, Addgene plasmid #126016; pET30-MBP-CfaN-His6, Addgene plasmid #126017; pET30-His6-Sumo-CfaC-MBP, Addgene plasmid #126018; pTXB1-His6-IntCtr-GyrA-His6, Addgene plasmid #126019. All other plasmids reported in this manuscript will be available upon request.

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Acknowledgements

The authors thank current members of the Muir laboratory (in particular G. Liszczak, M. Haugbro, A. Burton and J. Gramespacher) for discussions and comments. The authors also thank T. Srikumar of Princeton University Mass Spectrometry Facility. This work was supported by National Institutes of Health (NIH) grants R37 GM086868 and P01 CA196539. R.E.T. was supported by a Charles H. Revson Foundation postdoctoral fellowship and A.J.S. by a National Science Foundation graduate research fellowship (DGE-1148900). We dedicate this paper to R.T. Raines on the occasion of his 60th birthday.

Author information

R.E.T. and T.W.M. conceived the project. R.E.T., A.J.S. and T.W.M. designed all experiments and analysed all data. R.E.T. performed all experiments. R.E.T. and T.W.M. wrote the manuscript.

Correspondence to Tom. W. Muir.

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Supplementary information

Supplementary Information

The Supplementary Information file contains methods, protein sequences and characterization, as well as supplementary figures and data.

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