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Robots command enzymes

Enzymatic approaches to synthesize oligosaccharides offer an alternative to chemical syntheses for the production of homogeneous glycans; however, enzyme-based routes typically require lengthy processes. Now, the design of a water-soluble affinity tag has enabled the automation of multistep enzymatic syntheses of mammalian oligosaccharides.

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  1. 1.

    Li, T. et al. Nat. Chem. (2019).

  2. 2.

    Wen, L. et al. Chem. Rev. 118, 8151–8187 (2018).

  3. 3.

    Matsushita, T. et al. J. Am. Chem. Soc. 132, 16651–16656 (2010).

  4. 4.

    Tang, S.-L. & Pohl, N. L. Org. Lett. 17, 2642–2645 (2015).

  5. 5.

    Zhang, J. et al. Angew. Chem. Int. Ed. 57, 16638–16642 (2018).

  6. 6.

    Panza, M., Pistorio, S. G., Stine, K. J. & Demchenko, A. V. Chem. Rev. 118, 8105–8150 (2018).

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Correspondence to Nicola L. B. Pohl.

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Fig. 1: The water-soluble affinity tag enables the automation of multiple enzymatic reactions.