Abstract

Photochromic fluorescent proteins play key roles in super-resolution microscopy and optogenetics. The light-driven structural changes that modulate the fluorescence involve both trans-to-cis isomerization and proton transfer. The mechanism, timescale and relative contribution of chromophore and protein dynamics are currently not well understood. Here, the mechanism of off-to-on-state switching in dronpa is studied using femtosecond-to-millisecond time-resolved infrared spectroscopy and isotope labelling. Chromophore and protein dynamics are shown to occur on multiple timescales, from picoseconds to hundreds of microseconds. Following excitation of the trans chromophore, a ground-state primary product is formed within picoseconds. Surprisingly, the characteristic vibrational spectrum of the neutral cis isomer appears only after several tens of nanoseconds. Further fluctuations in protein structure around the neutral cis chromophore are required to form a new intermediate, which promotes the final proton-transfer reaction. These data illustrate the interplay between chromophore dynamics and the protein environment underlying fluorescent protein photochromism.

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Acknowledgements

S.R.M. acknowledges EPSRC for financial support (EP/N033647/1 and EP/M001997/1). P.J.T. acknowledges NSF for financial support (CHE-1223819). A.M. acknowledges the Japan Ministry of Education, Culture, Sports, Science and Technology Grant-in-aid for Scientific research on Innovative Areas: Resonance Bio. The authors acknowledge STFC for access to the Central Laser Facility. Calculations were performed on the High Performance Computing Cluster at the University of East Anglia.

Author information

Author notes

    • Sergey P. Laptenok

    Present address: Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia

    • Agnieszka A. Gil

    Present address: Department of Molecular Biology, Princeton University, Princeton, NJ, USA

    • Christopher R. Hall

    Present address: ARC Centre of Excellence in Exciton Science, School of Chemistry, The University of Melbourne, Parkville, Victoria, Australia

Affiliations

  1. School of Chemistry, University of East Anglia, Norwich, UK

    • Sergey P. Laptenok
    • , Christopher R. Hall
    • , Garth A. Jones
    •  & Stephen R. Meech
  2. Department of Chemistry, Stony Brook University, Stony Brook, NY, USA

    • Agnieszka A. Gil
    • , James N. Iuliano
    •  & Peter J. Tonge
  3. Department of Biophysics, Medical School, University of Pecs, Pecs, Hungary

    • Andras Lukacs
  4. Central Laser Facility, Harwell Science and Innovation Campus, Didcot, Oxfordshire, UK

    • Gregory M. Greetham
    •  & Paul Donaldson
  5. Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science, Wako, Saitama, Japan

    • Atsushi Miyawaki

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Contributions

S.P.L., A.A.G., C.R.H., A.L. and J.N.I. measured and collected the data. S.P.L. and C.R.H. analysed the data. A.A.G. and J.N.I. grew and purified the samples. G.A.J. performed the DFT calculations. G.M.G. and P.D. built, developed and managed the ULTRA and LifeTime apparatus used in the measurements. A.M. designed the dronpa2 protein. P.J.T. and S.R.M. designed the experiment and wrote the paper, with discussion and editorial input from all authors

Competing interests

The authors declare no competing interests.

Corresponding authors

Correspondence to Peter J. Tonge or Stephen R. Meech.

Supplementary information

  1. Supplementary information

    Supplementary Figures 1–9, Supplementary Tables 1–5, Supplementary Methods, Supplementary Data, Supplementary Analysis

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DOI

https://doi.org/10.1038/s41557-018-0073-0