Genetic clearance of p16high senescent cells or the use of senolytics improved the efficacy of stem cell reprogramming in vitro and in vivo, and helped establish induced pluripotent stem cells with features of experimental totipotency. When ablation of p16high senescent cells was combined with partial four-factor reprogramming in vivo, we observed noticeable histopathological liver rejuvenation in aged mice.
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This is a summary of: Grigorash, B. B. et al. p16High senescence restricts cellular plasticity during somatic cell reprogramming. Nat. Cell Biol. https://doi.org/10.1038/s41556-023-01214-9 (2023).
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Depletion of p16high senescent cells for stem cell reprogramming and tissue rejuvenation. Nat Cell Biol 25, 1252–1253 (2023). https://doi.org/10.1038/s41556-023-01216-7