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The beauty of simplicity in membrane biology

Nature Cell Biology volume 24, pages 1682–1685 (2022)Cite this article

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For the past 40 years, minimal reconstituted systems have helped cell biologists to understand the mechanisms that underlie membrane traffic. Having progressed from minimal synthetic and cell-derived ensembles to direct comparison with living systems, reconstitution is poised for ever more precise and informative understanding of membrane biology.

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Fig. 1: Methods and classes of reconstitution in membrane biology and traffic.

References

  1. Palade, G. Science 189, 867 (1975).

    Article  CAS  Google Scholar 

  2. Rothman, J. E. Angew. Chem. Int. Edn Engl. 53, 12676–12694 (2014).

    Article  CAS  Google Scholar 

  3. Skehel, J. J. & Wiley, D. C. Annu. Rev. Biochem. 69, 531–569 (2000).

    Article  CAS  Google Scholar 

  4. Schekman, R. Genes and Proteins that Control the Secretory Pathway (Nobel Lecture; 7 December 2013).

  5. Haas, A. & Wickner, W. EMBO J. 15, 3296–3305 (1996).

    Article  CAS  Google Scholar 

  6. Weber, T. et al. Cell 92, 759–772 (1998).

    Article  CAS  Google Scholar 

  7. Sutton, R. B., Fasshauer, D., Jahn, R. & Brunger, A. T. Nature 395, 347–353 (1998).

    Article  CAS  Google Scholar 

  8. Sudhof, T. C. Angew. Chem. Int. Edn Engl. 53, 12696–12717 (2014).

    Article  Google Scholar 

  9. Diao, J. et al. Nat. Protocols 7, 921–934 (2012).

    Article  CAS  Google Scholar 

  10. Wu, M. et al. Nat. Cell Biol. 12, 902–908 (2010).

    Article  CAS  Google Scholar 

  11. Mossman, K. D., Campi, G., Groves, J. T. & Dustin, M. L. Science 310, 1191–1193 (2005).

    Article  CAS  Google Scholar 

  12. Renard, H. F., Johannes, L. & Morsomme, P. Trends Cell Biol. 28, 274–286 (2018).

    Article  CAS  Google Scholar 

  13. Schoneberg, J. et al. Science 362, 1423–1428 (2018).

    Article  Google Scholar 

  14. Pfitzner, A. K. et al. Cell 182, 1140–1155 e18 (2020).

    Article  CAS  Google Scholar 

  15. Fotin, A. et al. Nature 432, 573–579 (2004).

    Article  CAS  Google Scholar 

  16. Faini, M. et al. Science 336, 1451–1454 (2012).

    Article  CAS  Google Scholar 

  17. Stagg, S. M. et al. Cell 134, 474–484 (2008).

    Article  CAS  Google Scholar 

  18. Dannhauser, P. & Ungewickell, E. Nat. Cell Biol. 14, 634 (2012).

    Article  CAS  Google Scholar 

  19. Floyd, D. L., Ragains, J. R., Skehel, J. J., Harrison, S. C. & van Oijen, A. M. Proc. Natl Acad. Sci. USA 105, 15382–15387 (2008).

    Article  CAS  Google Scholar 

  20. Hatzakis, N. S. et al. Nat. Chem. Biol. 5, 835–841 (2009).

    Article  CAS  Google Scholar 

  21. Bocking, T., Aguet, F., Harrison, S. C. & Kirchhausen, T. Nat. Struct. Mol. Biol. 18, 295–301 (2011).

    Article  CAS  Google Scholar 

  22. Antonny, B. et al. EMBO J. 35, 2270–2284 (2016).

    Article  CAS  Google Scholar 

  23. Stachowiak, J. et al. Nat. Cell Biol. 14, 944 (2012).

    Article  CAS  Google Scholar 

  24. Aguet, F. et al. Mol. Biol. Cell 27, 3418–3435 (2016).

    Article  CAS  Google Scholar 

  25. Kovtun, O., Dickson, V. K., Kelly, B. T., Owen, D. J. & Briggs, J. A. G. Sci. Adv. 6, eaba8381 (2020).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors acknowledge the support of the National Institutes of Health (NIH) through grants R35GM139531(J.C.S.), R01GM075252 (T.K.), and R35GM130386 (T.K.) and the Welch Foundation through grant F-2047 (J.C.S.).

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Authors and Affiliations

  1. University of Texas at Austin, Department of Biomedical Engineering, Austin, TX, USA

    Jeanne C. Stachowiak

  2. Harvard Medical School, Department of Cell Biology, Boston, MA, USA

    Tomas Kirchhausen

  3. Harvard Medical School, Department of Pediatrics, Boston, MA, USA

    Tomas Kirchhausen

Authors
  1. Jeanne C. Stachowiak
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  2. Tomas Kirchhausen
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Correspondence to Jeanne C. Stachowiak.

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The authors declare no competing interests.

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Stachowiak, J.C., Kirchhausen, T. The beauty of simplicity in membrane biology. Nat Cell Biol 24, 1682–1685 (2022). https://doi.org/10.1038/s41556-022-01015-6

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