a, The validated exosomal markers and exclusion markers candidates in exosomes purified using the three isolation methods in the three cell lines analysed. Protein abundance is expressed as log2-transformed SILAC ratios (exosome/super-SILAC standard) and the individual quantification values are depicted in the heat maps. Results from three biological replicates. b, STRING-based PPI network of the 22 ubiquitous and abundant exosomal proteins. c, Enriched GOCC, GOMF, GOBP, KEGG, Pfam and InterPro categories in the 22 exosomal marker candidates. d, Enriched GOMF, Pfam and InterPro categories in the cohort of low abundant exosomal proteins. e, Representation of the putative exosomal markers identified in our study. f, Representation of the putative exclusion markers identified in our study.