Extended Data Fig. 4: β-catenin binds multiple immune checkpoint gene loci; low-dose DXR has differential effects on IC genes in LSCs and blast cells. | Nature Cell Biology

Extended Data Fig. 4: β-catenin binds multiple immune checkpoint gene loci; low-dose DXR has differential effects on IC genes in LSCs and blast cells.

From: Overcoming Wnt–β-catenin dependent anticancer therapy resistance in leukaemia stem cells

Extended Data Fig. 4

a, CHIP-seq was performed on 2×107 cells from the β-catenin-3xFlag mouse ES cell line. Genome browser view of β-catenin binding density at the Pdcd1 (Pd-1), Havcr2 (Tim-3), Cd24a, and Ctla4 gene loci promoter regions and/or intergenic regions. b, ATAC-seq was used to show chromatin accessibility profiles of Wnt target genes observed in blast cells (~30k cells per replicate) and leukemic stem cells (~15k cells per replicate). c, Accessibility profiles of immuno-checkpoint genes observed by ATAC-seq in blast cells (~30k cells per replicate) and LSCs (~15k cells per replicate). Cells were sorted from BM pooled from 20 leukemia mice treated with low-dose DXR and 8 leukemia mice treated with vehicle control (b-c). Experiment was repeated 1 time with similar results.

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