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STEM CELLS

Engineering human knock-in organoids

Nature Cell Biology volume 22, pages 261–263 (2020)Cite this article

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The lack of endogenous reporter lines is a bottleneck in the study of subcellular dynamics in human adult stem cell (ASC)-derived organoids. An approach using CRISPR–Cas9-mediated homology-independent organoid transgenesis (CRISPR–HOT) in ASC-derived organoids now narrows the gap between basic research and translational studies in human organoids.

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Fig. 1: Workflow and cellular applications of CRISPR–HOT in ASC-derived organoids.

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Author notes

  1. These authors contributed equally: Qiutan Yang, Koen C. Oost.

Authors and Affiliations

  1. Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland

    Qiutan Yang, Koen C. Oost & Prisca Liberali

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  1. Qiutan Yang
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  2. Koen C. Oost
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  3. Prisca Liberali
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Correspondence to Prisca Liberali.

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Yang, Q., Oost, K.C. & Liberali, P. Engineering human knock-in organoids. Nat Cell Biol 22, 261–263 (2020). https://doi.org/10.1038/s41556-020-0478-z

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