CENP-ALAP bound DNAs at G1 and G2 were sequenced, with 2 replicates per condition, and mapped to the centromeric reference models in the hg38 assembly2,3. Shown are the raw mapping data (coloured) for every human centromere (except for the centromere of chromosome 19 that shares almost all of its α-satellites arrays with α-satellites arrays of chromosomes 1 and 5) and CENP-A binding called as SICER peaks (black lines, underneath) for one replicate for each time point. The experiment was repeated twice independently with similar results. Centromere reference location, red. CENP-B box, orange.