Supplementary Figure 7: The CpG status of promoters is involved in m6A methylation. | Nature Cell Biology

Supplementary Figure 7: The CpG status of promoters is involved in m6A methylation.

From: The RNA N6-methyladenosine modification landscape of human fetal tissues

Supplementary Figure 7

a. m(6)A distribution on mouse gene Ankrd9 and Zbtb42. Normalized reads densities of IP (orange) versus input (gray) were shown. b. RNA expression level of POU5F1/EEF1A1-Ankrd9/Zbtb42 in Hela cells. The expression level of Ankrd9 or Zbtb42 was first normalize to ACTB, and then relative to that with EEF1A1 promoters. Error bars represent mean s.d. from three independent experiments (see source data in Supplementary Table 5). c. Protein level of Ankrd9/Zbtb42, ACTB, IRF1, SMAD7 and GAPDH in promoter-displaced Hela cells. MYC tag placed before Ankrd9 or Zbtb42 coding regions was used to determine the protein level of them. Unprocessed data is shown in Supplementary Figure 8. The western blot was performed twice with similar results, and a representative figure was shown. d. Expression level of POU5F1/EEF1A1-Ankrd9/Zbtb42 in HEK293 cells. The relative expression level of Ankrd9 or Zbtb42 was judged as aforementioned. Two independent experiments were shown (see source data in Supplementary Table 5). e. Protein level of Ankrd9/Zbtb42, ACTB, IRF1 and GAPDH in promoter-displaced HEK293 cells. Unprocessed data is shown in Supplementary Figure 8. The western blot was performed twice with similar results, and a representative figure was shown. f. The relative m6A levels of ACTB, Ankrd9 and Zbtb42 in promoter displaced HEK293 cells were firstly normalized to their own inputs and then relative to GAPDH in corresponding cells. The experiments were performed twice (see source data in Supplementary Table 5). g. Lifetime of Ankrd9, Zbtb42, and IRF1 in promoter-displaced HEK293 cells after actinomycin D treatment. The plotted points represented the individual data from two independent experiments (see source data in Supplementary Table 5). h. Depth of CEBPZ and SMAD2 ChIP-Seq fragments (per bp per peak) around CpG-rich or CpG-poor TSS regions.

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