Supplementary Figure 5: eQTL and GWAS SNPs are associated with fetal tissue m6As. | Nature Cell Biology

Supplementary Figure 5: eQTL and GWAS SNPs are associated with fetal tissue m6As.

From: The RNA N6-methyladenosine modification landscape of human fetal tissues

Supplementary Figure 5

a. Odds ratio of eQTL SNPs enriched in m(6)As across all HapMap SNPs in corresponding tissues. n = 682487.04, 978250.18, 699224.11, 676464.39, 765139.26, 765776.76, 1391369.53, 1392390.1, 1395465.56, 1397037.35, 1394019.17, 1393978.32, 1396518.69, 1399635.18, 1404775.28, 1400517.83, 1398627.95, 1395485.53, 1392218 bases from the top down, respectively, two-sided Fisher’s exact test, P < 0.05 (see source data in Supplementary Table 5). b. Distribution of eQTL SNPs located in m(6)As in heart, liver, lung, muscle, and stomach in the 5′ UTR, CDS, intron, stop codon, 3′ UTR, promoter, and intergenic regions. c. Representative eQTL SNP on gene WNT2B (left) and GWAS SNP on gene HNF1A-AS1 (right). Normalized reads densities of MeRIP-Seq and Input-Seq of different tissues are shown. Reads of Input-Seq and MeRIP-Seq were gray and colored, respectively. All biological replicates are shown. Levels were normalized by the number of reads in each sample. Range is shown at the right side of the ‘brain-1’ track. The MeRIP-Seq was performed two or three times for each tissue type as indicated, and all replicates were shown. d. Enrichment of IPed versus input RNA of SNP-associated m(6)A peaks was normalized to GAPDH. MeRIP-qPCR was performed once with three technical replicates obtained and shown (see source data in Supplementary Table 5).

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