Supplementary Figure 1: ALOX12 is critical for p53-mediated ferroptosis. | Nature Cell Biology

Supplementary Figure 1: ALOX12 is critical for p53-mediated ferroptosis.

From: ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway

Supplementary Figure 1

(a) Schematic diagram of human ALOX family proteins. (b) Q-PCR of ALOX family members from H1299 Tet-on p533KR cells transfected with control siRNA (ctrl) or a pool of ALOX family specific siRNAs as indicated corresponding to Fig. 1a; Error bars are mean ± s.d., n = 3 independent experiments. (c) Western blot analysis of U2OS control or ALOX12 crispr clones #4 and #5. The experiments were repeated twice, independently, with similar results. (d) U2OS control or ALOX12 crispr clone #5, from c, were pre-incubated with Nutlin (10uM) for 12h, then treated with Nutlin (10 uM) and TBH (300 uM) as indicated for 8h. Error bars are mean ± s.d., n = 3 independent experiments. (e) U2OS control or ALOX12 crispr clone #5, from c, were pre-incubated with Nutlin (10 uM) for 12h, then treated with Nutlin (10uM) and TBH (300uM) for indicated times. Error bars are mean ± s.d., n = 3 independent experiments. All P values (b,d,e) were calculated using two-tailed unpaired Student’s t-test. Detailed statistical tests are described in the Methods. Scanned images of unprocessed blots are shown in Supplementary Fig. 9. Raw data are provided in Supplementary Table 1.

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