Supplementary Figure 8: Further characterization of ALOX12-mediated ferroptosis. | Nature Cell Biology

Supplementary Figure 8: Further characterization of ALOX12-mediated ferroptosis.

From: ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway

Supplementary Figure 8

(a) Schematic diagram of the strategy to generate ACSL4 conditional knockout mice. (b) Validation of ACSL4 knockout by southern blot. The experiments were repeated twice, independently, with similar results. (c) Validation of ACSL4 knockout by genotyping. The experiments were repeated twice, independently, with similar results. (d) U2OS cells were pre-incubated with Nutlin (10 uM) for 12 h, then treated with Nutlin (10 uM), Paraquat (1 mM), Ferr-1 (2 uM), and ML-355 (4 uM) as indicated for 8 h. Quantification of cell death is shown. Error bars are mean ± s.d., n = 3 independent experiments . P values were calculated using two-tailed unpaired Student’s t-test. (e) Schematic diagram of human chromosome 17p13.1 (upper panel) and mouse chromosome 11 (lower panel). ALOX12 and p53 locations indicated with distance between the two genes; MB, megabase. Scanned images of unprocessed blots are shown in Supplementary Fig. 9. Raw data are provided in Supplementary Table 1.

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