Supplementary Figure 6: Interaction of SLC7A11 with ALOX family proteins. | Nature Cell Biology

Supplementary Figure 6: Interaction of SLC7A11 with ALOX family proteins.

From: ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway

Supplementary Figure 6

(a, b, c, d) Western blot analysis of the interaction between SLC7A11 and ALOX family proteins (a, b, c, d). 293T cells were co-transfected with indicated constructs, and the extract was analysed by Co-IP assays. The western blot experiments were repeated twice, independently, with similar results. (e, f) Western blot analysis of the protein levels of ALOX12, ALOX15 and SLC7A11 (e, f). H1299 cells were transfected with indicated constructs. The western blot experiments were repeated twice, independently, with similar results. (g) Schematic diagram of WT and mutant SLC7A11. (h) Western blot analysis for the interactions of ALOX12 with WT, SLC7A11∆N, SLC7A11∆C. The western blot experiments were repeated twice, independently, with similar results. (i) Western blot analysis of the interaction of ALOX12 with SLC7A11 ∆91-140. The western blot experiments were repeated twice, independently, with similar results. (j) The effects of ALOX12 activity by SLC7A11-wt and SLC7A11∆91-140. Quantification of cell death is shown. Error bars are mean ± s.d., n = 3 independent experiments. P values were calculated using two-tailed unpaired Student’s t-test. Scanned images of unprocessed blots are shown in Supplementary Fig. 9. Raw data are provided in Supplementary Table 1.

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