Supplementary Figure 2: Regulation of lipid peroxidation levels by p53. | Nature Cell Biology

Supplementary Figure 2: Regulation of lipid peroxidation levels by p53.

From: ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway

Supplementary Figure 2

(a) GPX4 activity is measured by glutathione peroxidase assay kit in U2OS cells with or without Nutlin (10uM) treatment. Error bars are mean ± s.d., n = 3 independent experiments. (b) Lipid peroxidation levels in U2OS cells treated with Nutlin (10uM) and TBH (300uM) were assessed by flow cytometry using C11-BODIPY dye (c) Quantification of lipid peroxidation levels (b) is shown. Error bars are mean ± s.d., n = 3 independent experiments. (d) Representative phase-contrast images of H1299 Tet-on p533KR GPX4 crispr cells transfected with GPX4 vectors. Ferr-1 (1uM) was then withdrew from the cells (Scale bars, 100µm). The experiments were repeated three times, independently, with similar results. (e) Lipid peroxidation levels in H1299 Tet-on p533KR GPX4 crispr cells transfected with GPX4 were assessed by flow cytometry using C11-BODIPY dye. The experiments were repeated three times, independently, with similar results. All P values (a,c) were calculated using two-tailed unpaired Student’s t-test. Raw data are provided in Supplementary Table 1.

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