News & Views | Published:

CRISPR

MicroRNAs tame CRISPR–Cas9

Nature Cell Biologyvolume 21pages416417 (2019) | Download Citation

Subjects

MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    Fabian, M. R., Sonenberg, N. & Filipowicz, W. Annu. Rev. Biochem. 79, 351–379 (2010).

  2. 2.

    Wang, X.-W. et al. Nat. Cell Biol. https://doi.org/10.1038/s41556-019-0292-7 (2019).

  3. 3.

    Bartel, D. P. Cell 136, 215–233 (2009).

  4. 4.

    Zhao, Y. et al. Cell 129, 303–317 (2007).

  5. 5.

    Lagos-Quintana, M. et al. Curr. Biol. 12, 735–739 (2002).

  6. 6.

    van Rooij, E. et al. Science 316, 575–579 (2007).

  7. 7.

    Fiedler, J. & Thum, T. Arterioscler. Thromb. Vasc. Biol. 33, 201–205 (2013).

  8. 8.

    Issler, O. & Chen, A. Nat. Rev. Neurosci. 16, 201–212 (2015).

  9. 9.

    Liao, Q., Wang, B., Li, X. & Jiang, G. Oncotarget 8, 3666–3682 (2017).

  10. 10.

    Feng, Y.-H. & Tsao, C.-J. Biomed. Rep. 5, 395–402 (2016).

  11. 11.

    Chavez, A. et al. Nat. Methods 12, 326–328 (2015).

  12. 12.

    Wang, N. et al. Nat. Commun. 10, 95 (2019).

  13. 13.

    Wu, J. C., Sundaresan, G., Iyer, M. & Gambhir, S. S. Mol. Ther. 4, 297–306 (2001).

  14. 14.

    Gilbert, L. A. et al. Cell 159, 647–661 (2014).

  15. 15.

    Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A. & Liu, D. R. Nature 533, 420–424 (2016).

Download references

Author information

Affiliations

  1. RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA

    • Karina Jouravleva
    •  & Phillip D. Zamore
  2. Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA, USA

    • Phillip D. Zamore

Authors

  1. Search for Karina Jouravleva in:

  2. Search for Phillip D. Zamore in:

Competing interests

The authors declare no competing interests.

Corresponding authors

Correspondence to Karina Jouravleva or Phillip D. Zamore.

About this article

Publication history

Published

Issue Date

DOI

https://doi.org/10.1038/s41556-019-0302-9

Newsletter Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing