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CRISPR

MicroRNAs tame CRISPR–Cas9

MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.

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Fig. 1: The miRNA-inducible CRISPR–Cas9 (MICR) platform can monitor miRNA activity or restrict the activity of Cas9 or its fusion protein derivatives to precisely defined cell types.

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Correspondence to Karina Jouravleva or Phillip D. Zamore.

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Jouravleva, K., Zamore, P.D. MicroRNAs tame CRISPR–Cas9. Nat Cell Biol 21, 416–417 (2019). https://doi.org/10.1038/s41556-019-0302-9

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