We developed exponentially amplified rolling circle amplification with CRISPR–Cas12a as a one-pot, isothermal assay for microRNA detection. This method has single-digit femtomolar sensitivity and single-nucleotide specificity, and can be deployed for point-of-care testing. The assay has been adapted for the microRNA profiling of extracellular vesicles, which is used in the diagnosis of pancreatic cancer.
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This is a summary of: Yan, H. et al. A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-023-01033-1 (2023).
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A highly sensitive and robust one-pot assay for the detection of microRNAs. Nat. Biomed. Eng 7, 1539–1540 (2023). https://doi.org/10.1038/s41551-023-01043-z
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DOI: https://doi.org/10.1038/s41551-023-01043-z
