Multiplex detection of two interacting Mycobacterium tuberculosis biomarkers on the surface of circulating extracellular vesicles, using a nanoplasmon-enhanced immunoassay, improves the diagnosis of tuberculosis in immunosuppressed children living with HIV.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 digital issues and online access to articles
$99.00 per year
only $8.25 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Dodd, P. J. et al. The global burden of tuberculosis mortality in children: a mathematical modelling study. Lancet Glob. Health 5, e898–e906 (2017). This review discusses the challenges and importance of diagnosing paediatric TB.
Njuguna, I. N. et al. Urgent versus post-stabilisation antiretroviral treatment in hospitalised HIV-infected children in Kenya (PUSH): a randomised controlled trial. Lancet HIV 5, e12–e22 (2018). This report describes the diagnostically challenging cohort of paediatric patients with TB analysed in this study.
Liu, C. et al. Quantification of circulating Mycobacterium tuberculosis antigen peptides allows rapid diagnosis of active disease and treatment monitoring. Proc. Natl Acad. Sci. USA 114, 3969–3974 (2017). This study indicates the potential of Mtb-derived serum biomarkers to diagnose TB, using a sophisticated immunoassay that is read by mass spectrometry.
Gaur, R. L. et al. LprG-Mediated Surface Expression of Lipoarabinomannan Is Essential for Virulence of Mycobacterium tuberculosis. PLoS Pathog. 10, e1004376 (2014). This article indicates how the two biomarkers used in our assay interact to mediate Mtb virulence.
Schorey, J. S., Cheng, Y. & McManus, W. R. Bacteria- and host-derived extracellular vesicles—two sides of the same coin? J. Cell Sci. 134, jcs256628 (2021). This review reports that EVs from Mtb-infected macrophages can carry Mtb-derived factors and enter the circulatory system.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Zheng, W. et al. Diagnosis of paediatric tuberculosis by optically detecting two virulence factors on extracellular vesicles in blood samples. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-022-00922-1 (2022).
Rights and permissions
About this article
Cite this article
Using extracellular vesicles in blood to diagnose paediatric tuberculosis. Nat. Biomed. Eng 6, 930–931 (2022). https://doi.org/10.1038/s41551-022-00933-y
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41551-022-00933-y
