A method connecting single-cell genomic, transcriptomic or proteomic profiles to functional cellular characteristics, especially time-varying phenotypic changes, would inform our understanding of cancer biology. We present functional single-cell sequencing (FUNseq) to address this need and describe how it might provide a unique way to unravel mechanisms that drive cancer.
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This is a summary of: You, L. et al. Linking the genotypes and phenotypes of cancer cells in heterogenous populations via real-time optical tagging and image analysis. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-022-00853-x (2022).
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Functional single-cell sequencing links dynamic phenotypes to their genotypes. Nat. Biomed. Eng 6, 501–502 (2022). https://doi.org/10.1038/s41551-022-00877-3