Fig. 1: A cluster-based approach for the optimization of mRNA-encapsulating LNPs. | Nature Biomedical Engineering

Fig. 1: A cluster-based approach for the optimization of mRNA-encapsulating LNPs.

From: Lipid nanoparticles for the inhalation of mRNA

Fig. 1

a, The multidimensional design space of nebulized formulations of LNPs can be defined by ‘chemical traits’ (three are schematically depicted), such as the molar fraction of cationic helper lipids or the structure of the PEG–lipid conjugates, and can be efficiently explored by first characterizing the stability and delivery efficiency in vivo (via luminescence levels from the lungs of mice that inhaled LNPs encapsulating mRNA encoding nanoluciferase) of combinations of LNP formulations at the ‘extremes’ of the space (indicated by the coloured circles). b, Promising ‘extreme’ formulations are then ‘expanded’ around their vicinity in chemical space and characterized, which informs the subsequent optimization steps. Figure reproduced with permission from ref. 4, Springer Nature Limited.

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