Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

CANCER DIAGNOSTICS

Enhanced DNA libraries for methylation analysis

An efficient protocol for the preparation of DNA libraries for the analysis of methylation patterns in cell-free DNA in plasma enhances the sensitivity of bisulfite sequencing for the early detection of lung cancer.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Fig. 1: Three library-preparation workflows for bisulfite sequencing.

References

  1. Heitzer, E. et al. Nat. Rev. Genet. 20, 71–88 (2019).

    Article  CAS  Google Scholar 

  2. Liu, M. C. et al. Ann. Oncol. 31, 745–759 (2020).

    Article  CAS  Google Scholar 

  3. Parikh, A. R. et al. Clin. Cancer Res. https://doi.org/10.1158/1078-0432.CCR-21-0410 (2021).

  4. Liang, N. et al. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-021-00746-5 (2021).

  5. Gansauge, M.-T. & Meyer, M. Nat. Protoc. 8, 737–748 (2013).

    Article  Google Scholar 

  6. Schumacher, C., Kurihara, L. & Cunningham, K. Nat. Meth. 12, v–vi (2015).

    Article  CAS  Google Scholar 

  7. The Cancer Genome Atlas Research Network et al. Nat. Genet. 45, 1113–1120 (2013).

  8. Guo, S. et al. Nat. Genet. 49, 635–642 (2017).

    Article  CAS  Google Scholar 

  9. Liu, X. et al. EBioMedicine 41, 345–356 (2019).

    Article  Google Scholar 

  10. Liu, Y. et al. Nat. Biotechnol. 37, 424–429 (2019).

    Article  CAS  Google Scholar 

  11. Vaisvila, R. et al. Preprint at https://www.biorxiv.org/content/10.1101/2019.12.20.884692v2 (2019).

  12. Schutsky, E. K. et al. Nat. Biotechnol. 36, 1083–1090 (2018).

    Article  CAS  Google Scholar 

  13. Shen, S. Y., Burgener, J. M., Bratman, S. V. & De Carvalho, D. D. Nat. Protoc. 14, 2749–2780 (2019).

    Article  CAS  Google Scholar 

  14. Shen, S. Y. et al. Nature 563, 579–583 (2018).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Imran S. Haque.

Ethics declarations

Competing interests

I.S.H. is a former employee of Freenome and a shareholder in the company.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Haque, I.S. Enhanced DNA libraries for methylation analysis. Nat Biomed Eng 5, 490–492 (2021). https://doi.org/10.1038/s41551-021-00750-9

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41551-021-00750-9

Search

Quick links

Nature Briefing: Cancer

Sign up for the Nature Briefing: Cancer newsletter — what matters in cancer research, free to your inbox weekly.

Get what matters in cancer research, free to your inbox weekly. Sign up for Nature Briefing: Cancer