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Enhanced DNA libraries for methylation analysis

An efficient protocol for the preparation of DNA libraries for the analysis of methylation patterns in cell-free DNA in plasma enhances the sensitivity of bisulfite sequencing for the early detection of lung cancer.

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Fig. 1: Three library-preparation workflows for bisulfite sequencing.


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Correspondence to Imran S. Haque.

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I.S.H. is a former employee of Freenome and a shareholder in the company.

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Haque, I.S. Enhanced DNA libraries for methylation analysis. Nat Biomed Eng 5, 490–492 (2021).

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