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Culturing homogeneous microtissues at scale

Nature Biomedical Engineering volume 4, pages 849–850 (2020)Cite this article

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Microwell arrays enable the culture of thousands of organoids with increased homogeneity, and facilitate high-content image-based analyses and high-throughput drug screens.

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Fig. 1: Microwell arrays increase the homogeneity of organoids cultured in them.

References

  1. Dutta, D., Heo, I. & Clevers, H. Trends Mol. Med. 23, 393–410 (2017).

    Article  CAS  PubMed  Google Scholar 

  2. Lancaster, M. A. & Knoblich, J. A. Science 345, 1247125 (2014).

    Article  PubMed  Google Scholar 

  3. Schutgens, F. & Clevers, H. Annu. Rev. Pathol. Mech. Dis. 15, 211–234 (2020).

    Article  CAS  Google Scholar 

  4. Fatehullah, A., Tan, S. H. & Barker, N. Nat. Cell Biol. 18, 246–254 (2016).

    Article  PubMed  Google Scholar 

  5. Ranga, A., Gjorevski, N. & Lutolf, M. P. Adv. Drug Deliv. Rev. 69–70, 19–28 (2014).

    Article  PubMed  Google Scholar 

  6. Gjorevski, N. et al. Nature 539, 560–564 (2016).

    Article  CAS  PubMed  Google Scholar 

  7. Sato, T. et al. Nature 459, 262–265 (2009).

    Article  CAS  PubMed  Google Scholar 

  8. Sato, T. et al. Gastroenterology 141, 1762–1772 (2011).

    Article  CAS  PubMed  Google Scholar 

  9. Mustata, R. C. et al. Cell Rep. 5, 421–432 (2013).

    Article  CAS  PubMed  Google Scholar 

  10. Gracz, A. D. et al. Nat. Cell Biol. 17, 340–349 (2015).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Gunasekara, D. B. et al. Anal. Chem. 90, 1941–1950 (2018).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Boehnke, K. et al. J. Biomol. Screen. 21, 931–941 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Brandenberg, N. et al. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-020-0565-2 (2020).

  14. Wang, Y. et al. Biomaterials 128, 44–55 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Pastuła, A. et al. Stem Cells Int. 2016, 3710836 (2016).

    Article  PubMed  Google Scholar 

  16. Drost, J. et al. Science 358, 234–238 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Puca, L. et al. Nat. Commun. 9, 2404 (2018).

    Article  PubMed  PubMed Central  Google Scholar 

Download references

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Authors and Affiliations

  1. Joint Department of Biomedical Engineering at UNC and NCSU, Chapel Hill, NC, USA

    R. Jarrett Bliton & Scott T. Magness

  2. Department of Cell Biology & Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Scott T. Magness

  3. Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Scott T. Magness

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  1. R. Jarrett Bliton
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  2. Scott T. Magness
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Correspondence to Scott T. Magness.

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Bliton, R.J., Magness, S.T. Culturing homogeneous microtissues at scale. Nat Biomed Eng 4, 849–850 (2020). https://doi.org/10.1038/s41551-020-00608-6

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