Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

LAB-ON-A-CHIP DEVICES

Immunomagnetic cell sorting

Nature Biomedical Engineering volume 3, pages 759–760 (2019)Cite this article

Subjects

For genome-wide screens and other applications that require the processing of a large number of cells, the immunomagnetic sorting of cells on a microfluidic chip is a scalable, rapid and cost-efficient alternative to fluorescence-activated cell sorting.

Since the completion of the Human Genome Project, one challenge in modern genetics is to link individual genes to the specific biological processes that they control. This can be done at high throughput via phenotype-to-genotype approaches. For example, forward genetic screens using pooled libraries perturb multiple genes in parallel, followed by the selection of the phenotype of choice, and then by the identification of genetic perturbations linked with the selected phenotype. Initially, genome-scale pooled screens in human cells used RNA interference (RNAi), which allows for the identification of genes contributing to a phenotype in a genome-wide manner and in virtually any cell type1. However, RNAi methods suffer from the incomplete knockdown of target genes and from widespread off-target effects, which confound the interpretation of the results of the screen2.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

Buy

All prices are NET prices.

Fig. 1: MICS versus FACS.

References

  1. Root, D. E. et al. Nat. Meth. 3, 715–719 (2006).

    Article  CAS  Google Scholar 

  2. Jackson, A. L. et al. Nat. Biotech. 21, 635–637 (2003).

    Article  CAS  Google Scholar 

  3. Cong, L. et al. Science 339, 819–823 (2013).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Shalem, O. et al. Science 343, 84–87 (2014).

    Article  CAS  PubMed  Google Scholar 

  5. Kong, X. et al. Nature 550, 270–274 (2017).

    Article  PubMed  PubMed Central  Google Scholar 

  6. Patel, S. J. et al. Nature 548, 537–542 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Burr, M. L. et al. Nature 549, 101–105 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Binek, A. et al. J. Proteome Res. 18, 169–181 (2019).

    CAS  PubMed  Google Scholar 

  9. Mair, B. et al. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-019-0454-8 (2019).

  10. Weissman, I. Ann. Rev. Immun. 34, 1–30 (2016).

    Article  CAS  Google Scholar 

  11. Tseng, D. et al. Proc. Natl Acad. Sci. USA 110, 11103–11108 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Logtenberg, M. E. W. et al. Nat. Med. 25, 612–619 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Uhlen, M. et al. Science 357, eaan2507 (2017).

    Article  PubMed  Google Scholar 

  14. Zhang, S.-Q. et al. Nat. Biotech. 36, 1156–1159 (2018).

    Article  CAS  Google Scholar 

  15. McDonald, E. R. et al. Cell 170, 577–592 (2017).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. New York Genome Center, New York, NY, USA

    Mateusz Legut & Neville E. Sanjana

  2. Department of Biology, New York University, New York, NY, USA

    Mateusz Legut & Neville E. Sanjana

Authors
  1. Mateusz Legut
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Neville E. Sanjana
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Neville E. Sanjana.

Ethics declarations

Competing interests

N.E.S. is an advisor for Vertex.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Legut, M., Sanjana, N.E. Immunomagnetic cell sorting. Nat Biomed Eng 3, 759–760 (2019). https://doi.org/10.1038/s41551-019-0459-3

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41551-019-0459-3

Search

Advanced search

Quick links

Nature Briefing: Cancer

Sign up for the Nature Briefing: Cancer newsletter — what matters in cancer research, free to your inbox weekly.

Get what matters in cancer research, free to your inbox weekly. Sign up for Nature Briefing: Cancer