Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:


Barcodes for subcellular protein localization

The localization of target proteins, at subcellular resolution, in fixed patient-derived tissues can now be achieved via antibodies conjugated with tetrahedral DNA nanostructures self-assembled in situ.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Barcodes for the profiling of the identity and localization of subcellular proteins.


  1. Scott, J. D. & Pawson, T. Science 326, 1220–1224 (2009).

    Article  CAS  Google Scholar 

  2. Butler, G. S. & Overall, C. M. Nat. Rev. Drug Discov. 8, 935–948 (2009).

    Article  CAS  Google Scholar 

  3. Hung, M.-C. & Link, W. J. Cell Sci. 124, 3381–3392 (2011).

    Article  CAS  Google Scholar 

  4. Wilhelm, M. et al. Nature 509, 582–587 (2014).

    Article  CAS  Google Scholar 

  5. Lundberg, E. & Borner, G. H. H. Nat. Rev. Mol. Cell Biol. 20, 285–302 (2019).

    Article  CAS  Google Scholar 

  6. Thul, P. J. et al. Science 356, eaal3321 (2017).

    Article  Google Scholar 

  7. Giesen, C. et al. Nat. Methods 11, 417–422 (2014).

    Article  CAS  Google Scholar 

  8. Adan, A., Alizada, G., Kiraz, Y., Baran, Y. & Nalbant, A. Crit. Rev. Biotechnol. 37, 163–176 (2017).

    Article  CAS  Google Scholar 

  9. Sundah, N. R. et al. Nat. Biomed. Eng. (2019).

    Article  CAS  Google Scholar 

  10. Peterson, V. M. et al. Nat. Biotechnol. 35, 936–939 (2017).

    Article  CAS  Google Scholar 

  11. Aebersold, R. et al. Nat. Chem. Biol. 14, 206–214 (2018).

    Article  CAS  Google Scholar 

  12. Schnell, U., Dijk, F., Sjollema, K. A. & Giepmans, B. N. G. Nat. Methods 9, 152–158 (2012).

    Article  CAS  Google Scholar 

  13. Mardis, E. R. Nat. Protoc. 12, 213–218 (2017).

    Article  CAS  Google Scholar 

  14. Davies, A. K. et al. Nat. Commun. 9, 3958 (2018).

    Article  Google Scholar 

  15. Franco-Serrano, L. et al. Nucleic Acids Res. 46, D645–D648 (2018).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Amy E. Herr.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Y., Moss, A., Tan, K. et al. Barcodes for subcellular protein localization. Nat Biomed Eng 3, 673–675 (2019).

Download citation

  • Published:

  • Issue Date:

  • DOI:


Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research