Review Article | Published:

Manufacturing of primed mesenchymal stromal cells for therapy

Nature Biomedical Engineeringvolume 3pages90104 (2019) | Download Citation

Abstract

Mesenchymal stromal cells (MSCs) for basic research and clinical applications are manufactured and developed as unique cell products by many different manufacturers and laboratories, often under different conditions. The lack of standardization of MSC identity has limited consensus around which MSC properties are relevant for specific outcomes. In this Review, we examine how the choice of media, cell source, culture environment and storage affects the phenotype and clinical utility of MSC-based products, and discuss the techniques better suited to prime MSCs with specific phenotypes of interest and the need for the continued development of standardized assays that provide quality assurance for clinical-grade MSCs. Bioequivalence between cell products and batches must be investigated rather than assumed, so that the diversity of phenotypes between differing MSC products can be accounted for to identify products with the highest therapeutic potential and to preserve their safety in clinical treatments.

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Acknowledgements

This work was supported by grants from the National Key Scientific Program (2017CB964902) to J.Q.Y. and J.Z.

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Affiliations

  1. Beijing Cancer Hospital, Translational Medicine, Beijing, China

    • James Q. Yin
    •  & Jun Zhu
  2. Roy J. Carver Department of Biomedical Engineering, Fraternal Order of Eagles Diabetes Research Center, The University of Iowa, Iowa City, IA, USA

    • James A. Ankrum

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Contributions

J.Q.Y., J.Z. and J.A. conceived of and wrote the manuscript. J.Q.Y. and J.Z. generated the figures.

Competing interests

J.Q.Y. was involved in the production of the rejuvenating MSCM media, marketed by Geno Biotechnology Co. in China. J.A. and J.Z. declare no competing interests.

Corresponding authors

Correspondence to James Q. Yin or James A. Ankrum.

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https://doi.org/10.1038/s41551-018-0325-8