Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

SEQUENCING TECHNOLOGY

Epigenetic profiling with ultralow DNA amounts

A microfluidic device powers DNA-methylome sequencing of CpG islands and promoter regions, with minimal sample loss.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Comparison of DNA input using MID-RRBS and RRBS workflows for DNA-methylation profiling.
Fig. 2: Pearson correlation coefficients in the CG methylation level between samples processed by MID-RRBS or a Zymo kit.

References

  1. Chen, Z., Li, S., Subramaniam, S., Shyy, J. Y. & Chien, S. Annu. Rev. Biomed. Eng. 19, 195–219 (2017).

    Article  CAS  PubMed  Google Scholar 

  2. Wu, H. & Zhang, Y. Cell 156, 45–68 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Cokus, S. J. et al. Nature 452, 215–219 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Gu, H. et al. Nat. Methods 7, 133–136 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Marabita, F. et al. Epigenetics 8, 333–346 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Lister, R. et al. Cell 133, 523–536 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Lister, R. et al. Nature 462, 315–322 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Meissner, A. et al. Nucleic Acids Res. 33, 5868–5877 (2005).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Gu, H. et al. Nat. Protoc. 6, 468–481 (2011).

    Article  CAS  PubMed  Google Scholar 

  10. Ma, S. et al. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-018-0204-3 (2018).

  11. Malatesta, P. et al. Development 127, 5253–5263 (2000).

    CAS  PubMed  Google Scholar 

  12. Kukushkina, V. et al. Sci. Rep. 7, 3916 (2017).

    Article  PubMed  PubMed Central  Google Scholar 

  13. Varley, K. et al. Genome Res. 23, 555–567 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Luo, C. et al. Science 357, 600–604 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Zhen Chen.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lin, FM., Chien, S. & Chen, Z. Epigenetic profiling with ultralow DNA amounts. Nat Biomed Eng 2, 146–147 (2018). https://doi.org/10.1038/s41551-018-0211-4

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41551-018-0211-4

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing