Stem-cell-based therapies hold considerable promise for regenerative medicine. However, acute donor-cell death within several weeks after cell delivery remains a critical hurdle for clinical translation. Co-transplantation of stem cells with pro-survival factors can improve cell engraftment, but this strategy has been hampered by the typically short half-lives of the factors and by the use of Matrigel and other scaffolds that are not chemically defined. Here, we report a collagen–dendrimer biomaterial crosslinked with pro-survival peptide analogues that adheres to the extracellular matrix and slowly releases the peptides, significantly prolonging stem cell survival in mouse models of ischaemic injury. The biomaterial can serve as a generic delivery system to improve functional outcomes in cell-replacement therapy.
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We would like to thank J. Tao for her assistance with the performance of the bindingassay detailed in Supplementary Fig. 16. We would also like to thank Stanford Bio-X (A.S.L.), the National Institutes of Health (grants HL133272 (J.C.W.), HL132875 (J.C.W.),113006 (J.C.W.), EB009035 (J.C.W.) and HL134830-01 (P.K.N.)) and California Institute of Regenerative Medicine (CIRM; grants DR2-05394 and RT3-07798 (J.C.W.)) for funding support forthis study.
The authors declare no competing financial interests.
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Lee, A.S., Inayathullah, M., Lijkwan, M.A. et al. Prolonged survival of transplanted stem cells after ischaemic injury via the slow release of pro-survival peptides from a collagen matrix. Nat Biomed Eng 2, 104–113 (2018). https://doi.org/10.1038/s41551-018-0191-4
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