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Biosensing: CRISPR-powered diagnostics

A CRISPR-associated nuclease that can promiscuously cleave RNAs enables a rapid and cheap test for the single-molecule detection and single-base discrimination of nucleic acids.

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Figure 1: CRISPR-based nucleic acid detection4.

References

  1. Urdea, M. et al. Nature S1, 73–79 (2006).

    Article  Google Scholar 

  2. Zhao, Y., Chen, F., Li, Q., Wang, L. & Fan, C. Chem. Rev. 115, 12491–12545 (2015).

    Article  CAS  Google Scholar 

  3. Liu, L. et al. Cell 168, 121–134 (2017).

    Article  CAS  Google Scholar 

  4. Gootenberg, J. S. et al. Science 356, 438–442 (2017).

    Article  CAS  Google Scholar 

  5. Jinek, M. et al. Science 337, 816–821 (2012).

    Article  CAS  Google Scholar 

  6. Cong, L. et al. Science 339, 819–823 (2013).

    Article  CAS  Google Scholar 

  7. Barrangou, R. & Doudna, J. A. Nat. Biotechnol. 34, 933–941 (2016).

    Article  CAS  Google Scholar 

  8. Nelles, D. A. et al. Cell 165, 488–496 (2016).

    Article  CAS  Google Scholar 

  9. Pardee, K. et al. Cell 165, 1255–1266 (2016).

    Article  CAS  Google Scholar 

  10. Abudayyeh, O. O. et al. Science 353, aaf5573 (2016).

    Article  Google Scholar 

  11. East-Seletsky, A. et al. Nature 538, 270–273 (2016).

    Article  CAS  Google Scholar 

  12. Fu, Y. et al. Nat. Biotechnol. 31, 822–826 (2013).

    Article  CAS  Google Scholar 

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Correspondence to Chunhai Fan or Hong-Yuan Chen.

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Zuo, X., Fan, C. & Chen, HY. Biosensing: CRISPR-powered diagnostics. Nat Biomed Eng 1, 0091 (2017). https://doi.org/10.1038/s41551-017-0091

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  • DOI: https://doi.org/10.1038/s41551-017-0091

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