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Gene delivery: Suddenly squeezed and shocked

A microfluidic device that integrates mechanical squeezing and electrical stimulation delivers DNA to the nucleus of cells at a rate of millions of cells per minute.

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Figure 1: Schematic of the delivery of large molecules, such as DNA, into the nucleus of cells through mechanical squeezing of the cell (which disrupts the plasma membrane), followed by electric-field disruption of the nuclear membrane.


  1. Stewart, M. P. et al. Nature 538, 183–192 (2016).

    Article  CAS  Google Scholar 

  2. Ding, X. et al. Nat. Biomed. Eng. 1, 0039 (2017).

  3. Sharei, A. et al. Proc. Natl Acad. Sci. USA 110, 2082–2087 (2013).

    Article  CAS  Google Scholar 

  4. Raab, M. et al. Science 352, 359–362 (2016).

    Article  CAS  Google Scholar 

  5. Denais, C. M. et al. Science 352, 353–358 (2016).

    Article  CAS  Google Scholar 

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Correspondence to Mehmet Toner.

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Toner, M. Gene delivery: Suddenly squeezed and shocked. Nat Biomed Eng 1, 0047 (2017).

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