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Gene delivery: Suddenly squeezed and shocked

A microfluidic device that integrates mechanical squeezing and electrical stimulation delivers DNA to the nucleus of cells at a rate of millions of cells per minute.

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Figure 1: Schematic of the delivery of large molecules, such as DNA, into the nucleus of cells through mechanical squeezing of the cell (which disrupts the plasma membrane), followed by electric-field disruption of the nuclear membrane.

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Correspondence to Mehmet Toner.

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Toner, M. Gene delivery: Suddenly squeezed and shocked. Nat Biomed Eng 1, 0047 (2017). https://doi.org/10.1038/s41551-017-0047

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