A chemically defined protocol requiring no animal-derived components allows for the easier derivation and enduring expansion of epicardial cells from human pluripotent stem cells.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 digital issues and online access to articles
$99.00 per year
only $8.25 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Sayed, N., Liu, C. & Wu, J. C. J. Am. Coll. Cardiol. 67, 2161–2176 (2016).
Bao, X. et al. Nat. Biomed. Eng. 1, 0003 (2016).
von Gise, A. & Pu, W. T. Circ. Res. 110, 1628–1645 (2012).
Merki, E. et al. Proc. Natl Acad. Sci. USA 102, 18455–18460 (2005).
Cai, C. L. et al. Nature 454, 104–108 (2008).
Perez-Pomares, J. M. & de la Pompa, J. L. Circ. Res. 109, 1429–1442 (2011).
Smith, C. L., Baek, S. T., Sung, C. Y. & Tallquist, M. D. Circ. Res. 108, e15–e26 (2011).
Singh, A. et al. Cell Rep. 15, 1384–1393 (2016).
Zhou, B. et al. J. Clin. Invest. 121, 1894–1904 (2011).
Huang, G. N. et al. Science 338, 1599–1603 (2012).
Smart, N. et al. Nature 474, 640–644 (2011).
Wei, K. et al. Nature 525, 479–485 (2015).
Gessert, S. & Kuhl, M. Circ. Res. 107, 186–199 (2010).
Witty, A. D. et al. Nat. Biotechnol. 32, 1026–1035 (2014).
Iyer, D. et al. Development 142, 1528–1541 (2015).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Paik, D., Wu, J. Stem cell culture: Simply derived epicardial cells. Nat Biomed Eng 1, 0015 (2017). https://doi.org/10.1038/s41551-016-0015
Published:
DOI: https://doi.org/10.1038/s41551-016-0015