Cotton rats (N = 6) were immunized twice with 25 μg mRNA/LNP vaccine or DS-Cav1 formulated with Adju-Phos®. Sera collected 2 weeks following the second immunization were assayed for DCA (white bar), PCA (light gray bar), or 4D7CA (dark gray bar). The IT50 for each animal is plotted, and the geometric mean and 95% confidence intervals is shown. The dotted line at IT50 = 20 indicates the LOD for the assay. Animals with no competing antibody titers were assigned a titer at the LOD. DCA, PCA and 4D7 competing titers were compared within each group by one-way ANOVA using Graph Pad Prism software. Differences between titers within each immunization group are shown (p < 0.05 = *, p < 0.01 = **, p < 0.001 = ***, p < 0.0001 = ****).