Fig. 5 | npj Vaccines

Fig. 5

From: Neutrophil swarming and extracellular trap formation play a significant role in Alum adjuvant activity

Fig. 5

Deletion of PAD4 affects the magnitude of the adaptive immune response induced by alum adjuvant. Antigen-specific T (OTII) and B cells (MD4) were adoptively transfered to PAD4ko and WT mice. a Five days after immunisation, PAD4ko mice had similar numbers of cells in dLNs; b however, the proportion of antigen-specific OTII T cells was significantly lower in PAD4ko compared with WT mice (*P < 0.05). c Analysis of antigen-specific B cells on day 7 after immunisation revealed a similar proportion of the B-cell population in PAD4ko mice were IgMa+ HEL-specific; d however, the fewer GL7+ FAS+ germinal centre B cells were observed (*P < 0.05) compared with WT control mice. e PAD4KO mice immunised with OVA/Alum show reduced OVA-specific IgG1 titres compared with WT littermates 14 days after immunisation. f Levels of antigen-specific IgG2c antibodies are undetectable in both strains of mouse. g, h Antigen-specific IgG1 and IgG2a responses were similar in WT and PAD4KO mice 14 days after immunisation with OVA/ISCOMATRIX. Levels of antigen-specific IgG2c antibodies are undetectable in both strains of mouse. Antibody analysis was performed on two to four mice per group, and data are representative of two to three experiments. *P<0.05, **P < 0.01, ***P < 0.001

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