Fig. 3 | npj Vaccines

Fig. 3

From: Neutrophil swarming and extracellular trap formation play a significant role in Alum adjuvant activity

Fig. 3

Alum induces neutrophil recruitment and swarming at the site of immunisation within 2 h. LysM-eGFP mice were injected with a OVA alone or b OVA/Alum 10 min prior to intravital MPLSM. While some neutrophil recruitment could be observed following injection of OVA, substantially more neutrophils were observed following OVA/Alum injection. c Tracking GFP+ neutrophils using a dragon plots demonstrated highly linear, directional movement in tissue and d this was quantified using analysis of turning angle. e This directional movement resulted in the formation of localised neutrophil ‘swarms’. f Immunisation with Alexa-647-labelled alum confirmed that the neutrophils were swarming around deposits of alum at the site of injection. Images shown are representative of three independent experiments

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