Fig. 2 | npj Regenerative Medicine

Fig. 2

From: Biomimetic heterogenous elastic tissue development

Fig. 2

Surface modification for biofunctionalisation; post processing of 3D printed scaffolds. a Diagrammatic illustration of post processing of 3D printed scaffold, with collagen as an example of ECM component, L-AME as example bioactive molecule, which were introduced with solution based pre-polymer acting as a ‘glue’. Subpanels i-iv indicate the order in which post-processing of scaffolds can be carried out to obtain a biofunctionalised 3D printed scaffold. b Cell interactions on 3D printed scaffold surface (i) DAPI Fluorescence staining (Blue) demonstrating presence of cells on the scaffold (stained in red). SEM images. (ii) a, b. (higher mag). HDF at day 3 on 3D printed TPU90 surface. c, d. (higher mag) HDF at day 3 seeded on 3D printed TPU90 surface functionalised with L-AME. Red arrow indicating L-AME pores on the scaffold. e, f. (higher mag) HDFs at day 3 seeded on 3D printed TPU90 surface functionalised with collagen. (iii) BEC (at day 14) seeded as a submerged culture on 3D printed TPU80 surface biofunctionalised with collagen and L-AME. a. Mucus synthesis indicated within red circle. b. a cell prominent with Microvilli

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