a Volcano plot showing transcriptional adaptations to biofilm growth comparing RCCS-biofilm to stationary phase bacilli (red, induced in biofilm; blue, repressed). b Dendrogram of three biological replicates of RCCS-biofilm alongside log phase and stationary phase controls. Hierarchical clustering of mean-centred significantly differentially expressed genes to highlight differences between RNA signatures. c Genes significantly induced by biofilm growth, detailing M. bovis BCG and M. tb identifiers. Ordered by BCG ID.