Fig. 3 | npj Biofilms and Microbiomes

Fig. 3

From: New melanocortin-like peptide of E. coli can suppress inflammation via the mammalian melanocortin-1 receptor (MC1R): possible endocrine-like function for microbes of the gut

Fig. 3

Antibody to MC1R blocks suppressant effect of MECO-1 and alpha-MSH. Murine macrophage-like RAW 264.7 cells were pretreated with specific antibodies (“anti-MC1R Ab”) against melanocortin-1 receptor (MC1R) for 10 min prior to addition of HMGB1 in the absence or presence of MECO-1 or alpha-MSH at 10−12 M. At 6 h, the TNF content of the cell-free medium was determined by ELISA, and expressed as mean ± SEM of two independent experiments performed in duplicate. ACTH at the same concentration gave results that were indistinguishable from those of alpha-MSH and MECO-1 (data not shown)

Back to article page